Isotopes are separated by?
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The six methods of isotope separation we have described so far (diffusion, distillation, centrifugation, thermal diffusion, exchange reactions, and electrolysis) have all been tried with some degree of success on either uranium or hydrogen or both.
Mass spectroscopy is the technique which involves separation of elements based on their atomic masses. As isotopes have had same atomic number but different mass numbers they can be separated using mass spectroscopic techniques.