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Liposome mediated gene transfer or Lipofection
In this technique, DNA enters the protoplasts due to endocytosis of liposomes, involving the following steps: (i) adhesion of the liposomes to the protoplast surface, (ii) fusion of liposomes at the site of adhesion and (iii) release of plasmids inside the cell.
Microinjection is one of the physical methods of gene transfer used to introduce DNA or other genetic materials directly into a cell using a small glass needle or micropipette. This method allows efficient transfer and integration of desired genes into the host cell’s genome.