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navya
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Asked: 2024-05-23T13:50:03+05:30 In:

An enzyme is assayed at an initial substrate concentration of 2 x 10-5M. In 6 minute, half of the substrate is used. The Km for the substrate is 2 x 10-3M. The value of k in minute is

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An enzyme is assayed at an initial substrate concentration of 2 x 10-5M. In 6 minute, half of the substrate is used. The Km for the substrate is 2 x 10-3M. The value of k in minute is

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  1. navya
    Curator

    How to determine Km and Vmax. Km and Vmax are determined by incubating the enzyme with varying concentrations of substrate; the results can be plotted as a graph of rate of reaction (v) against concentration of substrate ([S], and will normally yield a hyperbolic curve, as shown in the graphs above.

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