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The dilution rate, D is defined as(where F = volumetric flow rate, VR = total volume of culture in the reactor and μ specific growth rate)
The dilution rate, D is defined as(where F = volumetric flow rate, VR = total volume of culture in the reactor and μ specific growth rate)
The dilution rate is calculated by dividing the flow rate (how much media flows into the vessel per hour) by the culture volume. For example, using a volume of 300 ml a dilution rate of 0.1 means that 30 ml of media is added to the culture every hour.