Which of the following have been used for cryo-preservation of germplasm with 90% tissue survival and 10%plant regeneration that are used in international exchange of germplasm?

A cryoprotectant is a substance used to protect biological tissue from freezing damage (i.e. that due to ice formation). Arctic and Antarctic insects, fish and amphibians create cryoprotectants (antifreeze compounds and antifreeze proteins) in their bodies to minimize freezing damage during cold winter periods.