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The size of the virulent plasmid of Agrobacterium tumefaciens is
Agrobacterium tumefaciens cell contains a plasmid known as the Ti (tumor-inducing) plasmid (140–235 kb).
Agrobacterium tumefaciens cell contains a plasmid known as the Ti (tumor-inducing) plasmid (140–235 kb).
See lessThe size of the virulent plasmid of Agrobacterium tumefaciens is
Agrobacterium tumefaciens cell contains a plasmid known as the Ti (tumor-inducing) plasmid (140–235 kb).
Agrobacterium tumefaciens cell contains a plasmid known as the Ti (tumor-inducing) plasmid (140–235 kb).
See lessVirulence trait ofAgrobacterium tumefaciens is borne on
Agrobacterium tumefaciens, a soil-borne phytopathogenic bacterium, transfers a segment of its Ti (tumor-inducing) plasmid, called T-DNA (transferred DNA), to plants .
Agrobacterium tumefaciens, a soil-borne phytopathogenic bacterium, transfers a segment of its Ti (tumor-inducing) plasmid, called T-DNA (transferred DNA), to plants .
See lessPlant transformation vectors based on Agrobacterium can generally be divided into
The Agrobacterium binary vector system is derived from natural tumor-inducing (Ti) plasmids. Agrobacterium transfers a region of the Ti-plasmid known as the transfer DNA (T-DNA) into numerous plant species, where it is integrated into the host genome.
The Agrobacterium binary vector system is derived from natural tumor-inducing (Ti) plasmids. Agrobacterium transfers a region of the Ti-plasmid known as the transfer DNA (T-DNA) into numerous plant species, where it is integrated into the host genome.
See lessVir genes required for the T-DNA transfer and processing are located
Vir genes required for the T-DNA transfer and processing are located outside the T-DNA region. Explanation: The gram-negative bacteria named agrobacterium transfers the T-DNA on the plant genome.
Vir genes required for the T-DNA transfer and processing are located outside the T-DNA region. Explanation: The gram-negative bacteria named agrobacterium transfers the T-DNA on the plant genome.
See lessIn response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
A gene is a segment of DNA that provides the code to construct a protein or RNA molecule.
A gene is a segment of DNA that provides the code to construct a protein or RNA molecule.
See lessThe transfer of intermediate vectors into Agrobacterium are brought about by
Intermediate vectors are replicated in E. coli and are transferred into Agrobacterium by conjugation. They cannot replicate in A. tumefaciens and therefore, carry DNA segments homologous to the disarmed T-DNA to permit recombination to form a co-integrated T-DNA structure.
Intermediate vectors are replicated in E. coli and are transferred into Agrobacterium by conjugation. They cannot replicate in A. tumefaciens and therefore, carry DNA segments homologous to the disarmed T-DNA to permit recombination to form a co-integrated T-DNA structure.
See lessBecause of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
Agrobacterium as Gene Vector for Plant Genetic Engineering
Agrobacterium as Gene Vector for Plant Genetic Engineering
See lessT-DNA transfer and processing into plant genome requires products of which of the following genes?
RB T-DNA repeat: Right border repeat of T-DNA. Upon recognized by Ti plasmid in Agrobacterium, the region between the T-DNA border repeats is transferred to plant cells. pVS1 StaA: Stability protein from the plasmid pVS1. Essential for stable plasmid segregation in Agrobacterium.
RB T-DNA repeat: Right border repeat of T-DNA. Upon recognized by Ti plasmid in Agrobacterium, the region between the T-DNA border repeats is transferred to plant cells. pVS1 StaA: Stability protein from the plasmid pVS1. Essential for stable plasmid segregation in Agrobacterium.
See lessThe direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as
Transfer of T-DNA process requires expression of virulence (vir) genes which are induced by phenolic signal molecules (Gelvin, 2003).
Transfer of T-DNA process requires expression of virulence (vir) genes which are induced by phenolic signal molecules (Gelvin, 2003).
See less