Why is CO2 Incubator Used in Animal Cell Culture?

CO2 incubator is a primary essential instrument used in an animal cell culture laboratory. It is essential in creating a controlled environment. It creates a favourable physiological environment for the animal cells to grow in-vitro. Animal cells are highly sensitive and require precise temperature, humidity, and carbon dioxide levels to survive, grow, and function properly outside the body.
The CO2 incubator maintains adequate pH in the culture media. Cell culture media consist of a bicarbonate buffering system. This involves a series of chemical reactions. When CO2 in an incubator interacts with the medium it creates unstable carbonic acid. This quickly breaks into bicarbonate ions and hydrogen ions, which balance the pH.